pLŒoÜ`(O. It is also available as a solution (Cat. Invitrogen Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. Hoechst 34580 possesses a dimethylamine group instead of the phenol, which shifts its emission maximum to 490 nm compared to the 461 nm of Hoechst 33258 and Hoechst 33342… Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Hoechst 33342, also known as bisbenzimide, is a blue fluorescent dye with high affinity for double-stranded DNA, especially those with AT-rich sequences. MDL number … Hoechst 33342 is a cell permeable benzimidazole dye that stains DNA by binding to the minor groove of adenine and thymine rich sequences It emits blue fluorescence excitation 350 nm : Buy from Supplier : Hoechst 33258 (Enzo Life Sciences) N/A : Buy from Supplier : Hoechst 33258 N/A. Application A fluorescent DNA stain qualified for use in Mycoplama staining. Cultured human neonatal dermal fibroblasts were fixed and permeabilized, with staining for tubulin (red fluorescence), actin (green fluorescence), and nuclei (blue-fluorescent, Spectroscopy, Elemental & Isotope Analysis, Preclinical to Companion Diagnostic Development, Chromatography Columns, Resins, & Spin Filters, CyQUANT Direct Microplate Reagent for Cell Viability, HCS LIVE/DEAD® Green Kit using HCS NuclearMask™ Deep Red, HCS LIVE/DEAD® Green Kit using Hoechst 33342, LIVE BacLight™ Bacterial Gram Stain Kit, LIVE/DEAD® Sperm Viability Kit Flow Cytometry, LIVE/DEAD® Sperm Viability Kit—Imaging, LIVE/DEAD® Viability/Cytotoxicity Kit for Mammalian Cells, NucGreen® Dead 488 ReadyProbes® Reagent for Viability, NucRed Dead 647 ReadyProbes Reagent Protocol for Viability, PrestoBlue HS and PrestoBlue Cell Viability Protocol, for Microplates, PrestoBlue® and CyQUANT® Direct Confirmation Assay for Cell Viability, ReadyProbes® Cell Viability Imaging Kit, Blue/Green, ReadyProbes® Cell Viability Imaging Kit, Blue/Red, LIVE/DEAD® BacLight™ Bacterial Viability Kit, LIVE/DEAD® Violet Viability/Vitality Kit, Vybrant® MTT Cell Proliferation Assay Kit, HCS NuclearMask™ Deep Red Stain Protocol, Hoechst® 33342 Protocol for HCA Instruments, ActinGreen™ 488 ReadyProbes® Reagent Protocol, ActinRed™ 555 ReadyProbes® Reagent Protocol, NucBlue® Live ReadyProbes® Reagent Protocol, NucBlue® Fixed Cell ReadyProbes® Reagent Protocol, NucRed® Dead 647 ReadyProbes® Reagent Protocol for Fixed Cells, NucRed® Live 647 ReadyProbes® Reagent Protocol, SYTOX® Green Nucleic Acid Stain Protocol, NucGreen® Dead 488 ReadyProbes® Reagent Protocol for Fixed Cells, BestProtocols: Annexin V Staining Protocol for Flow Cytometry, BestProtocols: BrdU Staining Protocol for Flow Cytometry, BestProtocols: Cell Preparation for Flow Cytometry Protocols, BestProtocols: UltraComp Compensation Beads Protocols for Flow Cytometry, BestProtocols: Pharmacological Induction of Apoptosis with Camptothecin, BestProtocols: Staining Cells with eFluor Proliferation Dyes for Flow Cytometry, BestProtocols: Red Blood Cell Lysis Protocols Using eBioscience Lysis Buffers, BestProtocols: Staining Cell Surface Targets for Flow Cytometry, BestProtocols: Staining Intracellular Antigens for Flow Cytometry, BestProtocols: Viability Staining Protocol for Flow Cytometry, BestProtocols: Immunofluorescent Staining of Intracellular Antigens on Cultured Cells, BestProtocols: IHC Frozen Tissue—Direct Method, BestProtocols: IHC Frozen Tissue—Indirect Method (purified), BestProtocols: IHC Frozen Tissue—Indirect Method (biotin), BestProtocols: IHC FFPE Tissue Proteolytic-Induced Epitope Retrieval—Direct Method, BestProtocols: IHC FFPE Tissue Trypsin Digestion Antigen Retrieval—Indirect Method, BestProtocols: IHC FFPE Tissue Low pH Antigen Retrieval—Direct Method, BestProtocols: IHC FFPE Tissue Low pH Antigen Retrieval—Indirect Method, BestProtocols: IHC FFPE Tissue High pH Antigen Retrieval—Direct Method, BestProtocols: IHC FFPE Tissue High pH Antigen Retrieval—Indirect Method, BestProtocols: Immunohistochemical Staining of Formalin-Fixed Paraffin-Embedded Tissues, BestProtocols: Colorimetric FFPE—High pH Antigen Retrieval, BestProtocols: Colorimetric FFPE—Low pH Antigen Retrieval, BestProtocols: Colorimetric FFPE—Trypsin Digestion, BestProtocols: ICC Formaldehyde Fixed Cells—Direct Method, BestProtocols: ICC Formaldehyde Fixed Cells—Indirect Method, BestProtocols: ICC Formaldehyde Fixed, Permeabilized Cells—Direct Method, BestProtocols: ICC Formaldehyde Fixed, Permeabilized Cells—Indirect Method, BestProtocols: ICC Methanol Fixed Cells—Direct Method, BestProtocols: ICC Methanol Fixed Cells—Indirect Method, BestProtocols: ICC Unfixed Cells—Direct Method. H1399), Nucleic acid (nuclear) staining in fluorescence microscopy. Hoechst 33342 is used for specifically staining the nuclei of living or fixed cells and tissues. Hoechst 33258 belongs to the family of Hoechst stains, which are a group of fluorescent stains for labeling DNA in fluorescence microscopy. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Hoechst 33258 dye concentrations to below 1 mg/ml has been suggested as a means of decreasing background fluorescence and thus improving the detection of lower DNA concentrations (3). It also has been used to detect DNA in solution between 250ng/ml to 20µg/ml in solution. Unbound dye has its maximum fluorescence emission in the … Our data show that Hoechst 33258, Hoechst 33342, and methylproamine also form complexes of the third type that reflects binding of dye associates (probably tetramers) to DNA. PURPOSE: The DNA-minor-groove-ligands bisbenzimidazoles Hoechst 33258 and 33342 have been reported to protect against radiation-induced DNA-strand breakage.In order to elucidate the mechanisms of protection by these DNA-binding compounds, pulse radiolysis studies on the reactions of the OH radical, the solvated electron and the H atom with Hoechst … No. BrdU incorporation into DNA has the ability to quench the fluorescence signal generated by Hoechst 33342 or Hoechst 33258 (4-7). The compound is cell permeable and is often used to stain live eukaryotic and bacterial cells. No. Hoechst dyes are often used as substitutes for another nucleic acid stain c… Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria. Hoechst 33528 is slightly more water soluble than Hoechst 33342, but both dyes are highly cell membrane permeant and widely used in cell cycle studies and as nuclear counterstains for live or fixed cells. Two of these closely related bis-benzimides are commonly used: Hoechst 33258 and Hoechst 33342… Molecular Weight 533.88 (anhydrous basis) EC Number 245-690-6. This bisbenzimide dye, Hoechst 34580, is related to the important minor-groove binding, dsDNA-selective dyes Hoechst 33258 and Hoechst 33342. Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak. However, the potential health hazard must be considered, as most nucleic acid stains are mutagenic. This stain can be used on both live and fixed cells (1). Hoechst dye is a known mutagen and should be handled with care. Figure 2. In order to elucidate the mechanisms of protection by these DNA-binding compounds, pulse radiolysis studies on the reactions of the OH radical, the solvated electron and the H atom with Hoechst … Cells are stained for 1-30 min at room temperature or 37 °C and then washed to remove unbound dye. Thermo Fisher Scientific. Bucket with ice Preparation of stock solutions of HO342, PI, and 7-AAD HO342 … Anticorps, Protéines recombinantes, coffret ELISA, RNAi, clones ADNc, Antibody Array, coffret Luminex. The Effect of Hoechst 33258 … Storage: The lyophilized powder is stable for 1 year if kept below 0ºC with desiccant. Staining with a low concentration (100 ng/ml) for 45 min. ’a=CoeëW’¹¤vSŒqBò “p…³4Ko®¿ß–#Ç܄ºÝ) C?†Ð±z6Ñ¥ü’¢pEƒ*3j«vy×)=mɛ2°JªZ'û°^¾w¥tíëe´„FÄ£ºnPìšýˆbĵÅh°=…óSñÑ?ƒ÷,vò’üöú†&‰ðϜYr,˜(à½óR¬Ž˜ìSE"“*”&6Ÿº HY-15631-100mg | Hoechst 33258 analog 6. *Please select more than one item to compare Previous message: Waldenstrom Next message: Wiley 15% Discount on PFC4 Messages sorted by: No. In order to optimize our detection limits, we sought to examine the effect of dye concentration on the fluorescent signal obtained over a range of DNA concentrations. Hoechst 33342, while closely related to Hoechst 33258, has been … Compared to Hoechst 33258, Hoechst 33342 is significantly more cell-permeable due to the addition of a lipophilic ethyl group and is usually preferred for living cell staining. Hoechst has very slow diffusion through thick tissue for instance, so for in vivo imaging applications (or in tissue sections), DAPI often stains viable cells much better than the (much heavier) 33342 because it is better able to diffuse through tissue. For Research … No. Its uses are similar to Hoechst 33342 for counterstaining, apoptosis and cell cycle studies, but Hoechst 33258 is reportedly less cell-permeant. Compare Products: Select up to 4 products. Hoechst 33258 is a cell permeable … These include Hoechst 33342, Hoechst 33258, 4′,6-diamidino-2-phenylindole (DAPI), mithramycin, propidium iodide, 7-amino-actinomycin D, SYTOX green, DRAQ5, and TO-PRO-3 iodide. —]Ì䄳ÑR¡e›@,óp–Ÿb=©H7،‰´=FÀlZ)TiRDÛ,e(LOÙ»"*‚b¿=T$UR¬b&B{$&ˆ”4»W)ã{!$×Wë. Organicville Dressing Reviews, Max Number Of Premier Balls Pokémon Go, Cody Jinks | The Wanting, Shifts In The Supply Curve Worksheet Answer Key, How To Pronounce Accordion, Uml Activity Diagram Object, Bumble And Bumble Oil, Blow Dry Spray, Worx Wg291 56v 24 Cordless Hedge Trimmer, Sony Mdr-v6 Discontinued, Black Hole On Earth, Battle For The Pride Lands Transcript, " /> pLŒoÜ`(O. It is also available as a solution (Cat. Invitrogen Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. Hoechst 34580 possesses a dimethylamine group instead of the phenol, which shifts its emission maximum to 490 nm compared to the 461 nm of Hoechst 33258 and Hoechst 33342… Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Hoechst 33342, also known as bisbenzimide, is a blue fluorescent dye with high affinity for double-stranded DNA, especially those with AT-rich sequences. MDL number … Hoechst 33342 is a cell permeable benzimidazole dye that stains DNA by binding to the minor groove of adenine and thymine rich sequences It emits blue fluorescence excitation 350 nm : Buy from Supplier : Hoechst 33258 (Enzo Life Sciences) N/A : Buy from Supplier : Hoechst 33258 N/A. Application A fluorescent DNA stain qualified for use in Mycoplama staining. Cultured human neonatal dermal fibroblasts were fixed and permeabilized, with staining for tubulin (red fluorescence), actin (green fluorescence), and nuclei (blue-fluorescent, Spectroscopy, Elemental & Isotope Analysis, Preclinical to Companion Diagnostic Development, Chromatography Columns, Resins, & Spin Filters, CyQUANT Direct Microplate Reagent for Cell Viability, HCS LIVE/DEAD® Green Kit using HCS NuclearMask™ Deep Red, HCS LIVE/DEAD® Green Kit using Hoechst 33342, LIVE BacLight™ Bacterial Gram Stain Kit, LIVE/DEAD® Sperm Viability Kit Flow Cytometry, LIVE/DEAD® Sperm Viability Kit—Imaging, LIVE/DEAD® Viability/Cytotoxicity Kit for Mammalian Cells, NucGreen® Dead 488 ReadyProbes® Reagent for Viability, NucRed Dead 647 ReadyProbes Reagent Protocol for Viability, PrestoBlue HS and PrestoBlue Cell Viability Protocol, for Microplates, PrestoBlue® and CyQUANT® Direct Confirmation Assay for Cell Viability, ReadyProbes® Cell Viability Imaging Kit, Blue/Green, ReadyProbes® Cell Viability Imaging Kit, Blue/Red, LIVE/DEAD® BacLight™ Bacterial Viability Kit, LIVE/DEAD® Violet Viability/Vitality Kit, Vybrant® MTT Cell Proliferation Assay Kit, HCS NuclearMask™ Deep Red Stain Protocol, Hoechst® 33342 Protocol for HCA Instruments, ActinGreen™ 488 ReadyProbes® Reagent Protocol, ActinRed™ 555 ReadyProbes® Reagent Protocol, NucBlue® Live ReadyProbes® Reagent Protocol, NucBlue® Fixed Cell ReadyProbes® Reagent Protocol, NucRed® Dead 647 ReadyProbes® Reagent Protocol for Fixed Cells, NucRed® Live 647 ReadyProbes® Reagent Protocol, SYTOX® Green Nucleic Acid Stain Protocol, NucGreen® Dead 488 ReadyProbes® Reagent Protocol for Fixed Cells, BestProtocols: Annexin V Staining Protocol for Flow Cytometry, BestProtocols: BrdU Staining Protocol for Flow Cytometry, BestProtocols: Cell Preparation for Flow Cytometry Protocols, BestProtocols: UltraComp Compensation Beads Protocols for Flow Cytometry, BestProtocols: Pharmacological Induction of Apoptosis with Camptothecin, BestProtocols: Staining Cells with eFluor Proliferation Dyes for Flow Cytometry, BestProtocols: Red Blood Cell Lysis Protocols Using eBioscience Lysis Buffers, BestProtocols: Staining Cell Surface Targets for Flow Cytometry, BestProtocols: Staining Intracellular Antigens for Flow Cytometry, BestProtocols: Viability Staining Protocol for Flow Cytometry, BestProtocols: Immunofluorescent Staining of Intracellular Antigens on Cultured Cells, BestProtocols: IHC Frozen Tissue—Direct Method, BestProtocols: IHC Frozen Tissue—Indirect Method (purified), BestProtocols: IHC Frozen Tissue—Indirect Method (biotin), BestProtocols: IHC FFPE Tissue Proteolytic-Induced Epitope Retrieval—Direct Method, BestProtocols: IHC FFPE Tissue Trypsin Digestion Antigen Retrieval—Indirect Method, BestProtocols: IHC FFPE Tissue Low pH Antigen Retrieval—Direct Method, BestProtocols: IHC FFPE Tissue Low pH Antigen Retrieval—Indirect Method, BestProtocols: IHC FFPE Tissue High pH Antigen Retrieval—Direct Method, BestProtocols: IHC FFPE Tissue High pH Antigen Retrieval—Indirect Method, BestProtocols: Immunohistochemical Staining of Formalin-Fixed Paraffin-Embedded Tissues, BestProtocols: Colorimetric FFPE—High pH Antigen Retrieval, BestProtocols: Colorimetric FFPE—Low pH Antigen Retrieval, BestProtocols: Colorimetric FFPE—Trypsin Digestion, BestProtocols: ICC Formaldehyde Fixed Cells—Direct Method, BestProtocols: ICC Formaldehyde Fixed Cells—Indirect Method, BestProtocols: ICC Formaldehyde Fixed, Permeabilized Cells—Direct Method, BestProtocols: ICC Formaldehyde Fixed, Permeabilized Cells—Indirect Method, BestProtocols: ICC Methanol Fixed Cells—Direct Method, BestProtocols: ICC Methanol Fixed Cells—Indirect Method, BestProtocols: ICC Unfixed Cells—Direct Method. H1399), Nucleic acid (nuclear) staining in fluorescence microscopy. Hoechst 33342 is used for specifically staining the nuclei of living or fixed cells and tissues. Hoechst 33258 belongs to the family of Hoechst stains, which are a group of fluorescent stains for labeling DNA in fluorescence microscopy. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Hoechst 33258 dye concentrations to below 1 mg/ml has been suggested as a means of decreasing background fluorescence and thus improving the detection of lower DNA concentrations (3). It also has been used to detect DNA in solution between 250ng/ml to 20µg/ml in solution. Unbound dye has its maximum fluorescence emission in the … Our data show that Hoechst 33258, Hoechst 33342, and methylproamine also form complexes of the third type that reflects binding of dye associates (probably tetramers) to DNA. PURPOSE: The DNA-minor-groove-ligands bisbenzimidazoles Hoechst 33258 and 33342 have been reported to protect against radiation-induced DNA-strand breakage.In order to elucidate the mechanisms of protection by these DNA-binding compounds, pulse radiolysis studies on the reactions of the OH radical, the solvated electron and the H atom with Hoechst … No. BrdU incorporation into DNA has the ability to quench the fluorescence signal generated by Hoechst 33342 or Hoechst 33258 (4-7). The compound is cell permeable and is often used to stain live eukaryotic and bacterial cells. No. Hoechst dyes are often used as substitutes for another nucleic acid stain c… Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria. Hoechst 33528 is slightly more water soluble than Hoechst 33342, but both dyes are highly cell membrane permeant and widely used in cell cycle studies and as nuclear counterstains for live or fixed cells. Two of these closely related bis-benzimides are commonly used: Hoechst 33258 and Hoechst 33342… Molecular Weight 533.88 (anhydrous basis) EC Number 245-690-6. This bisbenzimide dye, Hoechst 34580, is related to the important minor-groove binding, dsDNA-selective dyes Hoechst 33258 and Hoechst 33342. Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak. However, the potential health hazard must be considered, as most nucleic acid stains are mutagenic. This stain can be used on both live and fixed cells (1). Hoechst dye is a known mutagen and should be handled with care. Figure 2. In order to elucidate the mechanisms of protection by these DNA-binding compounds, pulse radiolysis studies on the reactions of the OH radical, the solvated electron and the H atom with Hoechst … Cells are stained for 1-30 min at room temperature or 37 °C and then washed to remove unbound dye. Thermo Fisher Scientific. Bucket with ice Preparation of stock solutions of HO342, PI, and 7-AAD HO342 … Anticorps, Protéines recombinantes, coffret ELISA, RNAi, clones ADNc, Antibody Array, coffret Luminex. The Effect of Hoechst 33258 … Storage: The lyophilized powder is stable for 1 year if kept below 0ºC with desiccant. Staining with a low concentration (100 ng/ml) for 45 min. ’a=CoeëW’¹¤vSŒqBò “p…³4Ko®¿ß–#Ç܄ºÝ) C?†Ð±z6Ñ¥ü’¢pEƒ*3j«vy×)=mɛ2°JªZ'û°^¾w¥tíëe´„FÄ£ºnPìšýˆbĵÅh°=…óSñÑ?ƒ÷,vò’üöú†&‰ðϜYr,˜(à½óR¬Ž˜ìSE"“*”&6Ÿº HY-15631-100mg | Hoechst 33258 analog 6. *Please select more than one item to compare Previous message: Waldenstrom Next message: Wiley 15% Discount on PFC4 Messages sorted by: No. In order to optimize our detection limits, we sought to examine the effect of dye concentration on the fluorescent signal obtained over a range of DNA concentrations. Hoechst 33342, while closely related to Hoechst 33258, has been … Compared to Hoechst 33258, Hoechst 33342 is significantly more cell-permeable due to the addition of a lipophilic ethyl group and is usually preferred for living cell staining. Hoechst has very slow diffusion through thick tissue for instance, so for in vivo imaging applications (or in tissue sections), DAPI often stains viable cells much better than the (much heavier) 33342 because it is better able to diffuse through tissue. For Research … No. Its uses are similar to Hoechst 33342 for counterstaining, apoptosis and cell cycle studies, but Hoechst 33258 is reportedly less cell-permeant. Compare Products: Select up to 4 products. Hoechst 33258 is a cell permeable … These include Hoechst 33342, Hoechst 33258, 4′,6-diamidino-2-phenylindole (DAPI), mithramycin, propidium iodide, 7-amino-actinomycin D, SYTOX green, DRAQ5, and TO-PRO-3 iodide. —]Ì䄳ÑR¡e›@,óp–Ÿb=©H7،‰´=FÀlZ)TiRDÛ,e(LOÙ»"*‚b¿=T$UR¬b&B{$&ˆ”4»W)ã{!$×Wë. 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hoechst 33258 vs 33342

For isolated cells however, Hoechst 33342 can much more rapidly stain viable nuclei (seconds vs… H3570). Hoechst 33258 Counterstaining with the chromosomal dye Hoechst 33258 is a simple procedure … The Hoechst stains may be used on live or fixed cells and are often used as a substitute for another nucleic acid stain (DAPI). Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Invitrogen Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. Background: Hoechst 33342 is a specific fluorescent DNA stain that binds within the minor groove of double-stranded AT-rich regions. xH 2 O . Hoechst stains property may allow fluorescence-activated cell and chromosome sorting according to the extent of DNA synthesis which may provide a bridge between biochemical and cytologic analyses of processes related to DNA replication. A concentration of 0.1–12 μg/ml is commonly used to stain DNA in bacteria or eukaryotecells. A combination of Hoechst 33342 … Propidium iodide (PI, e.g., Cat# 537059 EMD Millipore, MA) 5. Hoechst 33342 … They share many … It is also available as a solution (Cat. 7-Amino-actinomycin D (7-AAD, e.g., Cat #129935, EMD Millipore, MA) 6. In contrast, a Hoechst … Hoechst 34580 vs 33258, 33342 HawleyT@usa.redcross.org HawleyT at usa.redcross.org Wed Sep 3 12:22:03 EST 2003. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. A green fluorescence of unbound Hoechst dye may be observed on samples which are stained with too much dye or which are washed partially. Although Hoechst 33258 is shown here the emission spectra of Hoechst 33258 and 33342 are nearly identical (Molecular Probes, 1999–2000). Other investigators have utilized the ability of BrdU to reduce Hoechst fluorescence intensity to identify BrdU‐labeled cells and to differentiate the proportion of BrdU+ from BrdU− cells at … This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. Learn more >> For Research Use Only. Substitution of a hydrogen atom in the ortho position of the phenyl ring by a methyl group has a little effect on binding of monomers to DNA. The fluorescence signal from Hoechst dye is quenched by BrdU. Search results for hoechst 33342 at Sigma-Aldrich. However it reduces strength of binding of tetramers to DNA. Hoechst 34580 has somewhat longer-wavelength spectra than the other Hoechst dyes when bound to nucleic acids [Howard Shapiro, personal communication]. Hoechst 33342, trihydrochloride, trihydrate (Cat. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Hoechst 33258 nucleic acid stain is a popular nuclear counterstain that emits blue fluorescence when bound to dsDNA. a lipophilic ethyl group and is usually preferred for living cell staining. The key difference between them is that the additional ethyl group of Hoechst 33342 renders it more lipophilic, and thus more able to cross intact cell membranes. Search Unbound Hoechst dye has a maximum emission in the 510–540 nm range; a green haze may be observed if too much dye is applied. What we found was that: 1)Hoechst 34580 is much better excited by a violet laser than Hoechst 33342 or Hoechst 33258 (or DAPI). In some applications, Hoechst 33258 is … Components Contains 0.5μg/ml Hoechst bisbenzimide 33258 … Hoechst 33342 - CAS 23491-52-3 Catalog number: B2708-367394 Hoechst 33258, also called Pibenzimol, an iodinated DNA-binding bibenzimidazole, is a cell dye for DNA quantitation which sensitizes DNA and cells to UVA. Hoechst 33342 and Hoechst 33258 are structurally similar dyes that perform comparably as nuclear counterstains. 37°C water bath, centrifuge 7. Its uses are similar to Hoechst 33342 (H-1399) for counterstaining, apoptosis and cell cycle studies, but Hoechst 33258 is reportedly less cell-permeant. Hoechst … … Multicolor staining of human fibroblasts. 2)Hoechst 34580 gets into live cells and stains DNA stoichiometrically, as does Hoechst 33342, BUT 3)After a few days, or (sometimes) even a few hours in solution, Hoechst 34580 no longer produces anything remotely resembling a DNA histogram in either live cells or … This stain … I've successfully used Hoechst 33342 for live imaging of random cell migration in different types of breast cancer cell lines. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst 33342 binds preferentially to adenine-thymine (A-T) regions of DNA. The fact that Hoechst 33342 is not an intercalative dye makes it safer for general usage. Purpose : The DNA-minor-groove-ligands bisbenzimidazoles Hoechst 33258 and 33342 have been reported to protect against radiation-induced DNA-strand breakage. Hoechst 33342 (HO342, e.g., Cat# H3570 LifeTechnologies, Grand Island, NY) 4. Rechercher des fabricants et fournisseurs des Hoechst produits de Hoechst qualité supérieure Hoechst et à bon prix sur Alibaba.com This stain is commonly used in combination with 5-bromo-2'-deoxyuridine (BrdU) labeling to distinguish the compact chromatin of apoptotic nuclei, to identify replicating cells and to sort cells based on their DNA content. Hoechst 33342 (2'-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5'-bi-1H-benzimidazole trihydrochloride trihydrate) is a cell-permeable DNA stain that is excited by ultraviolet light and emits blue fluorescence at 460 to 490 nm. Aqueous solutions are stable for at least 1 month if kept in the … Dissolving Hoechst dye in PBS is not recommended, but phosphate-containing buffers may be used with dilute solutions of the dye. Compared to Hoechst 33258, Hoechst 33342 is significantly more cell-permeable due to the addition of. It is also available as a solution (Cat. H3570). ?Õ×ÄhH«gD"ôIv{ª¾¥žB–òÚ¨á’>pLŒoÜ`(O. It is also available as a solution (Cat. Invitrogen Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. Hoechst 34580 possesses a dimethylamine group instead of the phenol, which shifts its emission maximum to 490 nm compared to the 461 nm of Hoechst 33258 and Hoechst 33342… Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Hoechst 33342, also known as bisbenzimide, is a blue fluorescent dye with high affinity for double-stranded DNA, especially those with AT-rich sequences. MDL number … Hoechst 33342 is a cell permeable benzimidazole dye that stains DNA by binding to the minor groove of adenine and thymine rich sequences It emits blue fluorescence excitation 350 nm : Buy from Supplier : Hoechst 33258 (Enzo Life Sciences) N/A : Buy from Supplier : Hoechst 33258 N/A. Application A fluorescent DNA stain qualified for use in Mycoplama staining. Cultured human neonatal dermal fibroblasts were fixed and permeabilized, with staining for tubulin (red fluorescence), actin (green fluorescence), and nuclei (blue-fluorescent, Spectroscopy, Elemental & Isotope Analysis, Preclinical to Companion Diagnostic Development, Chromatography Columns, Resins, & Spin Filters, CyQUANT Direct Microplate Reagent for Cell Viability, HCS LIVE/DEAD® Green Kit using HCS NuclearMask™ Deep Red, HCS LIVE/DEAD® Green Kit using Hoechst 33342, LIVE BacLight™ Bacterial Gram Stain Kit, LIVE/DEAD® Sperm Viability Kit Flow Cytometry, LIVE/DEAD® Sperm Viability Kit—Imaging, LIVE/DEAD® Viability/Cytotoxicity Kit for Mammalian Cells, NucGreen® Dead 488 ReadyProbes® Reagent for Viability, NucRed Dead 647 ReadyProbes Reagent Protocol for Viability, PrestoBlue HS and PrestoBlue Cell Viability Protocol, for Microplates, PrestoBlue® and CyQUANT® Direct Confirmation Assay for Cell Viability, ReadyProbes® Cell Viability Imaging Kit, Blue/Green, ReadyProbes® Cell Viability Imaging Kit, Blue/Red, LIVE/DEAD® BacLight™ Bacterial Viability Kit, LIVE/DEAD® Violet Viability/Vitality Kit, Vybrant® MTT Cell Proliferation Assay Kit, HCS NuclearMask™ Deep Red Stain Protocol, Hoechst® 33342 Protocol for HCA Instruments, ActinGreen™ 488 ReadyProbes® Reagent Protocol, ActinRed™ 555 ReadyProbes® Reagent Protocol, NucBlue® Live ReadyProbes® Reagent Protocol, NucBlue® Fixed Cell ReadyProbes® Reagent Protocol, NucRed® Dead 647 ReadyProbes® Reagent Protocol for Fixed Cells, NucRed® Live 647 ReadyProbes® Reagent Protocol, SYTOX® Green Nucleic Acid Stain Protocol, NucGreen® Dead 488 ReadyProbes® Reagent Protocol for Fixed Cells, BestProtocols: Annexin V Staining Protocol for Flow Cytometry, BestProtocols: BrdU Staining Protocol for Flow Cytometry, BestProtocols: Cell Preparation for Flow Cytometry Protocols, BestProtocols: UltraComp Compensation Beads Protocols for Flow Cytometry, BestProtocols: Pharmacological Induction of Apoptosis with Camptothecin, BestProtocols: Staining Cells with eFluor Proliferation Dyes for Flow Cytometry, BestProtocols: Red Blood Cell Lysis Protocols Using eBioscience Lysis Buffers, BestProtocols: Staining Cell Surface Targets for Flow Cytometry, BestProtocols: Staining Intracellular Antigens for Flow Cytometry, BestProtocols: Viability Staining Protocol for Flow Cytometry, BestProtocols: Immunofluorescent Staining of Intracellular Antigens on Cultured Cells, BestProtocols: IHC Frozen Tissue—Direct Method, BestProtocols: IHC Frozen Tissue—Indirect Method (purified), BestProtocols: IHC Frozen Tissue—Indirect Method (biotin), BestProtocols: IHC FFPE Tissue Proteolytic-Induced Epitope Retrieval—Direct Method, BestProtocols: IHC FFPE Tissue Trypsin Digestion Antigen Retrieval—Indirect Method, BestProtocols: IHC FFPE Tissue Low pH Antigen Retrieval—Direct Method, BestProtocols: IHC FFPE Tissue Low pH Antigen Retrieval—Indirect Method, BestProtocols: IHC FFPE Tissue High pH Antigen Retrieval—Direct Method, BestProtocols: IHC FFPE Tissue High pH Antigen Retrieval—Indirect Method, BestProtocols: Immunohistochemical Staining of Formalin-Fixed Paraffin-Embedded Tissues, BestProtocols: Colorimetric FFPE—High pH Antigen Retrieval, BestProtocols: Colorimetric FFPE—Low pH Antigen Retrieval, BestProtocols: Colorimetric FFPE—Trypsin Digestion, BestProtocols: ICC Formaldehyde Fixed Cells—Direct Method, BestProtocols: ICC Formaldehyde Fixed Cells—Indirect Method, BestProtocols: ICC Formaldehyde Fixed, Permeabilized Cells—Direct Method, BestProtocols: ICC Formaldehyde Fixed, Permeabilized Cells—Indirect Method, BestProtocols: ICC Methanol Fixed Cells—Direct Method, BestProtocols: ICC Methanol Fixed Cells—Indirect Method, BestProtocols: ICC Unfixed Cells—Direct Method. H1399), Nucleic acid (nuclear) staining in fluorescence microscopy. Hoechst 33342 is used for specifically staining the nuclei of living or fixed cells and tissues. Hoechst 33258 belongs to the family of Hoechst stains, which are a group of fluorescent stains for labeling DNA in fluorescence microscopy. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Hoechst 33258 dye concentrations to below 1 mg/ml has been suggested as a means of decreasing background fluorescence and thus improving the detection of lower DNA concentrations (3). It also has been used to detect DNA in solution between 250ng/ml to 20µg/ml in solution. Unbound dye has its maximum fluorescence emission in the … Our data show that Hoechst 33258, Hoechst 33342, and methylproamine also form complexes of the third type that reflects binding of dye associates (probably tetramers) to DNA. PURPOSE: The DNA-minor-groove-ligands bisbenzimidazoles Hoechst 33258 and 33342 have been reported to protect against radiation-induced DNA-strand breakage.In order to elucidate the mechanisms of protection by these DNA-binding compounds, pulse radiolysis studies on the reactions of the OH radical, the solvated electron and the H atom with Hoechst … No. BrdU incorporation into DNA has the ability to quench the fluorescence signal generated by Hoechst 33342 or Hoechst 33258 (4-7). The compound is cell permeable and is often used to stain live eukaryotic and bacterial cells. No. Hoechst dyes are often used as substitutes for another nucleic acid stain c… Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria. Hoechst 33528 is slightly more water soluble than Hoechst 33342, but both dyes are highly cell membrane permeant and widely used in cell cycle studies and as nuclear counterstains for live or fixed cells. Two of these closely related bis-benzimides are commonly used: Hoechst 33258 and Hoechst 33342… Molecular Weight 533.88 (anhydrous basis) EC Number 245-690-6. This bisbenzimide dye, Hoechst 34580, is related to the important minor-groove binding, dsDNA-selective dyes Hoechst 33258 and Hoechst 33342. Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak. However, the potential health hazard must be considered, as most nucleic acid stains are mutagenic. This stain can be used on both live and fixed cells (1). Hoechst dye is a known mutagen and should be handled with care. Figure 2. In order to elucidate the mechanisms of protection by these DNA-binding compounds, pulse radiolysis studies on the reactions of the OH radical, the solvated electron and the H atom with Hoechst … Cells are stained for 1-30 min at room temperature or 37 °C and then washed to remove unbound dye. Thermo Fisher Scientific. Bucket with ice Preparation of stock solutions of HO342, PI, and 7-AAD HO342 … Anticorps, Protéines recombinantes, coffret ELISA, RNAi, clones ADNc, Antibody Array, coffret Luminex. The Effect of Hoechst 33258 … Storage: The lyophilized powder is stable for 1 year if kept below 0ºC with desiccant. Staining with a low concentration (100 ng/ml) for 45 min. ’a=CoeëW’¹¤vSŒqBò “p…³4Ko®¿ß–#Ç܄ºÝ) C?†Ð±z6Ñ¥ü’¢pEƒ*3j«vy×)=mɛ2°JªZ'û°^¾w¥tíëe´„FÄ£ºnPìšýˆbĵÅh°=…óSñÑ?ƒ÷,vò’üöú†&‰ðϜYr,˜(à½óR¬Ž˜ìSE"“*”&6Ÿº HY-15631-100mg | Hoechst 33258 analog 6. *Please select more than one item to compare Previous message: Waldenstrom Next message: Wiley 15% Discount on PFC4 Messages sorted by: No. In order to optimize our detection limits, we sought to examine the effect of dye concentration on the fluorescent signal obtained over a range of DNA concentrations. Hoechst 33342, while closely related to Hoechst 33258, has been … Compared to Hoechst 33258, Hoechst 33342 is significantly more cell-permeable due to the addition of a lipophilic ethyl group and is usually preferred for living cell staining. Hoechst has very slow diffusion through thick tissue for instance, so for in vivo imaging applications (or in tissue sections), DAPI often stains viable cells much better than the (much heavier) 33342 because it is better able to diffuse through tissue. For Research … No. Its uses are similar to Hoechst 33342 for counterstaining, apoptosis and cell cycle studies, but Hoechst 33258 is reportedly less cell-permeant. Compare Products: Select up to 4 products. Hoechst 33258 is a cell permeable … These include Hoechst 33342, Hoechst 33258, 4′,6-diamidino-2-phenylindole (DAPI), mithramycin, propidium iodide, 7-amino-actinomycin D, SYTOX green, DRAQ5, and TO-PRO-3 iodide. —]Ì䄳ÑR¡e›@,óp–Ÿb=©H7،‰´=FÀlZ)TiRDÛ,e(LOÙ»"*‚b¿=T$UR¬b&B{$&ˆ”4»W)ã{!$×Wë.

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