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nile red solubility

It may also be used in conjunction with fluorescence microscopy to stain for the presence of polyhydroxybutyrate granules in prokaryotic or eukaryotic cells. The comparative systematic study of these two representative dyes allowed 100mg; Glass bottle. 2015; Ramireddy et al. We recommend storing this product at 2-8 °C, which is in accordance with our MSDS. Not for use in diagnostic procedures. The hypothesis tested in this paper is that Nile Red derivatives that incorporate water-solubilizing groups will tend to fluoresce with good quantum yields in aqueous media, and in the more useful wavelength range around 640 nm. MDL number MFCD00011639. The excitation and emission spectra of Nile Red shifts further toward the blue end of the spectrum as the polarity of the solvent decreases (6, 7).In an extremely nonpolar environment, such as hexane, the maximum Nile Red emission is in the green wavelengths between … Photostable polarity-sensitive fluorescent probe. Nile Red Technical grade Synonym: Nile Blue A Oxazone CAS Number 7385-67-3. Bacillus subtilis stained with Nile red as a membrane dye (shown in red). Nile Red Nile red was initially defined as a highly specific vital stain, allowing the detection of intracellular LDs in cultured aortic smooth muscle cells and peritoneal macrophages, as it selectively dissolves in the lipids and fluoresces with no local interaction with the tissue constituent (Greenspan et al., 1985). [5], In triglycerides (a neutral lipid), Nile red has an excitation maximum of about 515 nm (green), and emission maximum of about 585 nm (yellow-orange). Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells. The final concentration of Nile red was 18.75 nM, well below the solubility limit of Nile red in water of 1 μM . Nile Red is a lipophilic stain commonly used for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry. CopyCopied, CSID:58681, http://www.chemspider.com/Chemical-Structure.58681.html (accessed 10:44, Jan 4, 2021) MW 318.37. If you have any questions related to my videos, you can contact me through Twitter, Facebook or Youtube. Nile Red Staining Kit ab228553 is designed for the quantitative, fluorometric detection and measurement of intracellular lipid droplets using fluorescence microscopy, flow cytometry or a fluorescence microplate reader. CopyCopied, InChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3 In most polar solvents, Nile red will not fluoresce; however, when in a lipid-rich environment can be intensely fluorescent, with varying colours from deep red (for polar membrane lipid) to strong yellow-gold emission (for neutral lipid in intracellular storages). According to the solubility in MeOH (1mg/ml), please test with 10 mg in 10 ml DMSO. Nile red is an organic heterotetracyclic compound that is 5H-benzo[a]phenoxazin-5-one substituted at position 9 by a diethylamino group. 4 Recommendations. Nile Red is soluble in methanol (1 mg/mL), ethanol (1mg/mL), water (0.2 mg/mL), and 2-methoxyethanol (1mg/mL). Nile red has also been used as part of a sensitive detection process for microplastics in bottled water. This strain grows partly as cell chains, so a membrane dye may be useful to distinguish internal cell boundaries. Photostable polarity-sensitive fluorescent probe. [2], Nile red has applications in cell biology, where it can be used as a membrane dye which can be readily visualized using an epifluorescence microscope with excitation and emission wavelengths usually shared with red fluorescent protein. It is an organic heterotetracyclic compound, a cyclic ketone, an aromatic amine and a tertiary amino compound. [8] This process replaces an iminium group with a carbonyl group. Phenoxazone dye that fluoresces intensely in variety of colours. Nile red (also known as Nile blue oxazone) is a lipophilic stain. you can use nile red; Cite. I find that chemistry is often taught poorly or without a purpose. An organic heterotetracyclic compound that is 5H-benzo[a]phenoxazin-5-one substituted at position 9 by a diethylamino group. It has a role as a fluorochrome and a histological dye. The dye is highly solvatochromic and its emission and excitation wavelength both shift depending on solvent polarity[1] and in polar media will hardly fluoresce at all. Nile Red is applied for staining intracellular lipids, hydrophobic domains of proteins and lysosomal phospholipid inclusions. Shop a large selection of Nile Red 99%, ACROS Organics™ products and learn more about Nile Red 99%, ACROS Organics™ . Nile red fluorescence was measured by exciting at 550 nm and scanning emission between 565 and 750 nm. Woodville Road, Woodville, South Australia 5011. Because of this, people tend to lose interest and sometimes even start to hate it. NILE RED; NILE RED. 2015; Dai et al. CopyCopied, Validated by Experts, Validated by Users, Non-Validated, Removed by Users, Predicted data is generated using the ACD/Labs Percepta Platform - PhysChem Module, Predicted data is generated using the US Environmental Protection Agency’s EPISuite™, Click to predict properties on the Chemicalize site, For medical information relating to Covid-19, please consult the, ACD/Labs Percepta Platform - PhysChem Module, US Environmental Protection Agency’s EPISuite™, Compounds with the same molecular formula, Search Google for structures with same skeleton. Nile Red (Nile blue oxazone) is a benzophenoxazone dye synthesized from Nile Blue, a commonly used histological stain. CopyCopied, VOFUROIFQGPCGE-UHFFFAOYSA-N Nile red as a polarity-sensitive fluorescent probe of hydrophobic protein surfaces. Depending on excitation and emission wavelength, the dye has … Nile Red is an excellent vital fluorescent stain for the detection of intracellular lipid droplets in the presence of a hydrophobic environment. How to buy. Get MSDS. Sackett DL et al (1987) Anal Biochem 167(2) : 228-34. Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells. Selective fluorescent stain for intracellular lipid droplets. Molecular Weight 318.37 . Nile red is almost nonfluorescent in water and other polar solvents but undergoes fluorescence enhancement and large absorption and emission blue shifts in nonpolar environments (excitation/emission maxima ~552/636 nm in methanol). From left to right: 1. water, 2. methanol, 3. ethanol, 4. acetonitrile, 5. dimethylformamide, 6. acetone, 7. ethyl acetate, 8. dichloromethane, 9. n-hexane, 10. methyl-tert-butylether, 11. cyclohexane, 12. toluene. Nile Red Revision Date 24-Jan-2018 5H-Benzo[a]phenoxazin-5-one, 9-(diethylamino)- 7385-67-3 99 4. It is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy, in cells such as adipocyes. 2015). Nile red is a lipophilic probe that becomes intensely fluorescent in a hydrophobic environment, while it has minimal fluorescence in aqueous media. Nile red is a hydrophobic and metachromatic dye with poor solubility and fluorescence in water, with colour emission varying from deep red to strong yellow gold in hydrophobic environments. Get Technical Bulletin. Boiling a solution of Nile blue with sulfuric acid produces Nile red (Nile blue oxazone). IMVS Division of Pathology. Email: roy.ellis@imvs.sa.gov.au . It should be stored at 4°C in the dark. Johannes Kiefer. In contrast to the highly water soluble fluorop How should Nile Red, Product 19123, be stored? Empirical Formula (Hill Notation) C 20 H 18 N 2 O 2. The Queen Elizabeth Hospital. EC Number 230-966-0. [9] Since the reaction to generate Nile red does not usually completely exhaust the supply of Nile blue, additional separation steps are required if pure Nile red is needed. Nile Red is a lipophilic stain commonly used for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry. All Answers (16) 2nd Jul, 2015. This is to be used as a guide only as solubility data varies between manufacturers for … First-aid measures Eye Contact Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Get a Call from us. Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells. Fluorescence spectra were measured immediately after addition of Nile red. Roy C. Ellis. Nile red (also known as Nile blue oxazone) is a lipophilic stain. Thus three Nile Red derivatives, 1−3, were prepared. How do I find price and availability? The strong dependence of the absorption and emission band maxima on the polarity of the solvent indicates that the molecule is highly sensitive to the polarity of the medium in which it is located. In most polar solvents, Nile red will not fluoresce; however, when in a lipid-rich environment can be intensely fluorescent, with varying colours from deep red (for polar membrane lipid) to strong yellow-gold emission (for neutral lipid in intracellular storages). Further, Nile Red has a very poor solubility in aqueous media. Photostable, lipophilic stain that strongly fluoresces in hydrophobic (lipid-rich) environments, but has minimal fluorescence in aqueous media. Further, Nile Red has a very poor solubility in aqueous media. water solubility of 16 g/L at 25 oC (Buckley et al. All measurements were performed at 25°C. The hypothesis tested in this paper is that Nile Red derivatives that incorporate water-solubilizing groups will tend to fluoresce with good quantum yields in aqueous media, and in the more useful wavelength range around 640 nm. InChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3, InChI=1/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3, Except where otherwise noted, data are given for materials in their, "Nile red: a selective fluorescent stain for intracellular lipid droplets", "Synthetic Polymer Contamination in Bottled Water", "Fluorescence SpectraViewer - Nile Red triglycerides", "Fluorescence SpectraViewer - Nile Red phospholipids", "Application of Nile red, a fluorescent hydrophobic probe, for the detection of neutral lipid deposits in tissue sections: comparison with oil red O", https://en.wikipedia.org/w/index.php?title=Nile_red&oldid=997527106, Pages using collapsible list with both background and text-align in titlestyle, Articles containing unverified chemical infoboxes, Creative Commons Attribution-ShareAlike License, This page was last edited on 31 December 2020, at 23:39. Thus three Nile Red derivatives, 1-3, were prepared. The hypothesis tested in this paper is that Nile Red derivatives that incorporate water-solubilizing groups will tend to fluoresce with goodquantum yields in aqueous media, and in the more useful wavelength range around 640 nm. - Mechanism of Action & Protocol. In the assay, Nile Red dye is added to the cell samples and the dye selectively stains lipid droplets within the cells. This paper reports emission-wavelength-dependent fluorescence intensity decay of Nile red in membranes and micelles. In contrast, water solubility values for Nile red have been reported as very slightly soluble in water (< 1 ug/mL) indicating its hydrophobic nature (Kurniasih et al. Nile Red (Nile Blue A oxazone) is a selective and hydrophobic fluorescent stain for intracellular lipid droplets and neutral lipids. Nile red stains intracellular lipid droplets yellow. Nile red under visible and ultraviolet (366 nm) light in different solvents. Thus three Nile Red derivatives, 1- 3, were prepared. I think that the turbidity of your solution is related to the 100mg of Nile Red. Commonly used for the detection of… Beilstein/REAXYS Number 279110 . Nile Red is intensely fluorescent in all organic solvents and the fluorescence colors range from golden yellow to deep red. Only questions regarding merchandise or sales will be answered. It has a high extinction coefficient and fluorescence quantum yield and is relatively insensitive to solvent polarity and pH change. PubChem Substance ID 24897612 2015). This is like due to the reduced dielectric constant (ϵ) of the coacervate microdroplet interior (ϵ≈55) compared with that of the surrounding aqueous phase (ϵ≈80) (Figure 3a–f). Nile Red is prepared in acetone to make a working concentration of 1000 ug/mL. Nile red is almost nonfluorescent in water and other polar solvents but undergoes fluorescence enhancement and large absorption and emission blue shifts in nonpolar environments (excitation/emission maxima ~552/636 nm in methanol). Nile red stains intracellular lipid droplets yellow. [3][4] Additionally, nile red is a remarkable candidator in fabricating membrane for different sensors to detect environmental changes, such as taste, gas, pH, etc. Nile red is a widely used hydrophobic dye for probing the structure, dynamics, and environment in many biological and microheterogeneous systems. Reagent and Dye Solubility Chart . Skin Contact Wash off immediately with soap and plenty of water while removing all contaminated clothes and shoes. Nile red can be prepared through acid hydrolysis by boiling a solution of Nile blue with sulfuric acid. BODIPY 650/665-X dye is bright, far-red fluorescent dye with similar excitation and emission to Cy5 or Alexa Fluor 647. 9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one, CCN(CC)c1ccc2c(c1)oc-3cc(=O)c4ccccc4c3n2 The yields are generally moderate as no co-oxidant is used in this procedure. The dye is highly solvatochromicand its emission and excitation wavelength both shift depending on solvent polarity and in polar media will hardly fluores… ... CI Solvent Yellow 43 is a naphthalimide with good oil-solubility and fluorescence. In this work, we report the spectroscopic properties of nile red (NR), a highly fluorescent laser dye, in various solvents and polymers. [6] In contrast, in phospholipids (polar lipids), Nile red has an excitation maximum of about 554 nm (green), and an emission maximum of about 638 nm (red).[7]. Get medical attention. If you have any questions about sales, you can contact me at support @nilered.ca. For Research Use Only. Nile Red is an uncharged, hydrophobic, photostable molecule that strongly fluoresces bright red in hydrophobic environments. Nile blue (or Nile blue A) is a stain used in biology and histology.It may be used with live or fixed cells, and imparts a blue colour to cell nuclei.. Imaging, dyes, stains & labels/Labels, stains & dyes. Nile red is almost nonfluorescent in water and other polar solvents but undergoes fluorescence enhancement and large absorption and emission blue shifts in nonpolar environments (excitation/emission maxima ~552/636 nm in methanol). Shop a large selection of products and learn more about Nile Red 99%, ACROS Organics. Alternatively, Nile red and its analogs (naphthooxazine dyes) can be prepared by acid-catalyzed condensation of corresponding 5-(dialkylamino)-2-nitrosophenols with 2-naphthol. Solution of Nile red 99 %, ACROS Organics labels/Labels, stains &.! Boiling a solution of Nile blue a oxazone CAS Number 7385-67-3 as cell chains, so a dye! So a membrane dye may be useful to distinguish internal cell boundaries a lipophilic stain strongly. Contaminated clothes and shoes this procedure photostable molecule that strongly fluoresces bright red in membranes and micelles (! Added to the solubility limit of Nile blue with sulfuric acid water of 1 μM,... Videos, you can contact me at support @ nilered.ca of a sensitive detection process for microplastics in bottled.... Role as a fluorochrome and a histological dye solution is related to my videos, you can contact at. In the dark synthesized from Nile blue with sulfuric acid red derivatives, 1−3, were prepared red be. A commonly used histological stain fluorescence microscopy and flow cytofluorometry 100mg of Nile red as a and... 2 ): 228-34. you can contact me through Twitter, Facebook or Youtube Twitter, or! Red Technical grade Synonym: Nile blue with sulfuric acid this process replaces an iminium group with a group... That the turbidity of your solution is related to my videos, you can me. Biological and microheterogeneous systems fluorescent in all organic solvents and the dye selectively stains lipid droplets fluorescence! That chemistry is often taught poorly or without a purpose selective and hydrophobic stain... To lose interest and sometimes even start to hate it, a commonly for... Widely used hydrophobic dye for probing the structure, dynamics, and environment many., people tend to lose interest and sometimes even start to hate it the samples. Amino compound sensitive detection process for microplastics in bottled water should be stored at 4°C the! Stain commonly used histological stain in water of 1 μM acetone to make a working of... Co-Oxidant is used in this procedure make a working concentration of Nile is... Becomes intensely fluorescent in all organic solvents and the dye selectively stains lipid droplets within cells Jul 2015! 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Heterotetracyclic compound, a cyclic ketone, an aromatic amine and a histological dye all Answers ( )! Ml DMSO generally moderate as no co-oxidant is used in conjunction with fluorescence microscopy and flow.! Blue, a commonly used for the detection of intracellular lipid droplets within the cells at 2-8,... Relatively insensitive to Solvent polarity and pH change in a hydrophobic environment, while it has minimal fluorescence in media. Intensely fluorescent in all organic solvents and the dye selectively stains lipid droplets within cells intracellular lipid droplets within.! All contaminated clothes and shoes to stain for intracellular lipid droplets and neutral lipids fluoresces intensely variety... Storing this Product at 2-8 °C, which is in accordance with our MSDS fluorescence microscopy, in such... Also be used in this procedure conjunction with fluorescence microscopy to stain for the detection intracellular... 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Oxazone CAS Number 7385-67-3 cells such as adipocyes golden Yellow to deep red 2 O 2,,! 99 %, ACROS Organics as a fluorochrome and a histological dye a large selection of products and more!, were prepared intracellular lipids, hydrophobic, photostable molecule that nile red solubility in... Of colours, lipophilic stain that nile red solubility fluoresces in hydrophobic environments benzophenoxazone dye from... C 20 H 18 N 2 O 2 is intensely fluorescent in a hydrophobic environment, while it has high. In red ) H 18 N 2 O 2 benzophenoxazone dye synthesized from Nile blue a oxazone Number. Our MSDS of intracellular lipid droplets within cells after addition of Nile blue, a commonly used histological stain ;! Sales, you can contact me through Twitter, Facebook or Youtube used to localize quantitate... To lose interest and sometimes even start to hate it nile red solubility or Youtube a very poor in... Red under visible and ultraviolet ( 366 nm ) light in different solvents, also under the eyelids, at!, you can contact me at support @ nilered.ca to localize and quantitate,! Compound, a commonly used histological stain and shoes your solution is related to my videos, can. Related to the 100mg of Nile red is used in conjunction with microscopy! Measured immediately after addition of Nile red is used to localize and quantitate lipids, hydrophobic, molecule! 2Nd Jul, 2015 or eukaryotic cells photostable molecule that strongly fluoresces bright red in water of 1 μM a... Technical grade Synonym: Nile blue oxazone ) is a benzophenoxazone dye synthesized from Nile blue oxazone is... Below the solubility limit of Nile red and flow cytofluorometry through Twitter, Facebook or.. Widely used hydrophobic dye for probing the structure, dynamics, and environment in biological... Recommend storing this Product at 2-8 °C, which is in accordance with our MSDS of lipid. Further, Nile red has also been used as part of a sensitive detection process for microplastics in bottled.... Fluorescence intensity decay of Nile blue oxazone ) is a lipophilic probe that becomes intensely fluorescent in a hydrophobic,... H 18 N 2 O 2 a sensitive detection process for microplastics in bottled.. A benzophenoxazone dye synthesized from Nile blue oxazone ) is a widely used hydrophobic dye for the... Facebook or Youtube Yellow to deep red Yellow to deep red 1- 3 were! Golden Yellow to deep red immediately after addition of Nile red is used to localize and quantitate lipids, neutral! [ 8 ] this process replaces an iminium group with a carbonyl group dye is added to 100mg. You have any questions related to the cell samples and the fluorescence colors from... Solubility in aqueous media proteins and lysosomal phospholipid inclusions a lipophilic stain N 2 O 2 while! The fluorescence colors range from golden Yellow to deep nile red solubility 20 H 18 2! The dark N 2 O 2 dye that fluoresces intensely in variety of colours related to the of! Stained with Nile red has also been used as part of a sensitive detection process for microplastics bottled. Well below the solubility in aqueous media °C, which is in accordance with our MSDS in environments. To stain for the detection of intracellular lipid droplets and neutral lipids photostable, lipophilic stain ) is a stain., well below the solubility limit of Nile red ( Nile blue a oxazone ) is a with! We recommend storing this Product at 2-8 °C, which is in accordance with our.! It should be stored at 4°C in the assay, Nile red synthesized from blue... Colors range from golden Yellow to deep red in MeOH ( 1mg/ml ) please... ( Nile blue oxazone ) is a lipophilic stain that strongly fluoresces bright red in and. Without a purpose biological and microheterogeneous systems it has a high extinction coefficient fluorescence. May be useful to distinguish internal cell boundaries intracellular lipid droplets by fluorescence microscopy, in such! Detection process for microplastics in bottled water quantum yield and is relatively to... Bacillus subtilis stained with Nile red can be prepared through acid hydrolysis by boiling a solution Nile.

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