The objective of this study was to evaluate the therapeutic effect of human adipose derived stem cells (hASCs) superimposed on a scleral contact lens (SCL) carrier in an ocular alkaline burn rabbit model.
After inducing alkaline burn in 11 New Zealand white rabbits, SCA-cultured hASCs were placed in the right eye of five rabbits, SCL without cells were used in five, and no treatment was applied to one eye. Each eye was examined and photographed for corneal vascularization, opacities and epithelial defect at week 1, 2 and 4 after surgery. After 1 month, the rabbits were killed and the corneas were removed and cut in half for electron and electron microscopy examinations.
Stem cells derived from human adipocytes were bound to the SCL surface and easily confluent. Stem cells derived from human adipose in SCL eyes presented less epithelial defect, less corneal opacity, corneal neovascularization in relation to SCL eyes. Both groups did not show simblefrão. However, the cornea in the untreated eye was melted in 2 weeks and developed severe syblepharon.
The researchers concluded that stem cells derived from human adipose in SCL can reduce inflammation and turbidity of the cornea in lesions of severe ocular alkaline burns in rabbits.
Espandar L, Caldwell D, Watson R, Blanco-Mezquita T, Zhang S, Bunnell B. Application of adipose-derived stem cells in the scleral contact lens carrier in an acute severe alkaline burning animal model. Contact lens with eyes. 2014 June 4.